High-throughput bacterial genome sequencing: an embarrassment of choice, a world of opportunity

Nat Rev Microbiol. 2012 Sep;10(9):599-606. doi: 10.1038/nrmicro2850. Epub 2012 Aug 6.


Here, we take a snapshot of the high-throughput sequencing platforms, together with the relevant analytical tools, that are available to microbiologists in 2012, and evaluate the strengths and weaknesses of these platforms in obtaining bacterial genome sequences. We also scan the horizon of future possibilities, speculating on how the availability of sequencing that is 'too cheap to metre' might change the face of microbiology forever.

Publication types

  • Comparative Study
  • Review

MeSH terms

  • DNA, Bacterial / chemistry*
  • DNA, Bacterial / genetics*
  • Genetics, Microbial / trends
  • Genome, Bacterial*
  • High-Throughput Screening Assays*
  • Molecular Biology / trends


  • DNA, Bacterial