Enhanced haloarchaeal oil removal in hypersaline environments via organic nitrogen fertilization and illumination

Extremophiles. 2012 Sep;16(5):751-8. doi: 10.1007/s00792-012-0471-y. Epub 2012 Aug 7.

Abstract

Hypersaline soil and pond water samples were mixed with 3 % crude oil, some samples were autoclaved to serve as sterile controls; experimental samples were not sterilized. After 6-week incubation at 40 °C under light/dark cycles, the soil microflora consumed 66 %, and after 4 weeks the pond water microflora consumed 63 % of the crude oil. Soil samples treated with 3 % casaminoacids lost 89 % of their oil after 6 weeks and water samples lost 86 % after 4 weeks. Samples treated with casaminoacids and antibiotics that selectively inhibited bacteria, lost even more oil, up to 94 %. Soil-water mixtures incubated under continuous illumination lost double as much more oil than samples incubated in the dark. The soil-water mixture at time zero contained 1.3 × 10(4) CFU g(-1) of hydrocarbon-utilizing microorganisms which were affiliated to Halomonas aquamarina, Exiguobacterium aurantiacum, Haloferax sp., Salinococcus sp., Marinococcus sp. and Halomonas sp. After 6-week incubation with oil, these numbers were 8.7 × 10(7) CFU g(-1) and the Haloferax sp. proportion in the total microflora increased from 20 to 93 %. Experiments using the individual cultures and three other haloarchaea isolated earlier from the same site confirmed that casaminoacids and light enhanced their oil consumption potential in batch cultures.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Archaea / growth & development*
  • Archaea / metabolism*
  • Biodegradation, Environmental
  • Nitrogen / metabolism
  • Petroleum / metabolism*
  • Petroleum / microbiology*
  • Petroleum Pollution*
  • Salinity
  • Soil Microbiology*
  • Water Microbiology

Substances

  • Petroleum
  • Nitrogen