Determination of 10 ginsenosides in Panax ginseng of different harvest times based on HPLC fingerprints and principal component analysis

Nat Prod Res. 2013;27(9):851-4. doi: 10.1080/14786419.2012.711758. Epub 2012 Aug 7.

Abstract

A combinative method of HPLC fingerprinting and quantitative determination was successfully applied to monitor dynamic accumulation of ginsenosides in five-year-old Panax ginseng roots from different harvest times. The optimal chromatographic conditions were achieved on a C18 column with gradient elution using acetonitrile and 1 mmol · L(-1) KH2PO4 buffer solution at 203 nm. The result indicated that the contents of total ginsenosides showed significant variations, and a decrease tendency appeared in the growth period of the fifth year.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetonitriles / chemistry
  • Chromatography, High Pressure Liquid / methods*
  • Ginsenosides / analysis*
  • Ginsenosides / chemistry
  • Panax / chemistry*
  • Plant Roots / chemistry
  • Principal Component Analysis
  • Reproducibility of Results

Substances

  • Acetonitriles
  • Ginsenosides
  • acetonitrile