Caenorhabditis elegans vulval cell fate patterning

Phys Biol. 2012 Aug;9(4):045001. doi: 10.1088/1478-3975/9/4/045001. Epub 2012 Aug 7.

Abstract

The spatial patterning of three cell fates in a row of competent cells is exemplified by vulva development in the nematode Caenorhabditis elegans. The intercellular signaling network that underlies fate specification is well understood, yet quantitative aspects remain to be elucidated. Quantitative models of the network allow us to test the effect of parameter variation on the cell fate pattern output. Among the parameter sets that allow us to reach the wild-type pattern, two general developmental patterning mechanisms of the three fates can be found: sequential inductions and morphogen-based induction, the former being more robust to parameter variation. Experimentally, the vulval cell fate pattern is robust to stochastic and environmental challenges, and minor variants can be detected. The exception is the fate of the anterior cell, P3.p, which is sensitive to stochastic variation and spontaneous mutation, and is also evolving the fastest. Other vulval precursor cell fates can be affected by mutation, yet little natural variation can be found, suggesting stabilizing selection. Despite this fate pattern conservation, different Caenorhabditis species respond differently to perturbations of the system. In the quantitative models, different parameter sets can reconstitute their response to perturbation, suggesting that network variation among Caenorhabditis species may be quantitative. Network rewiring likely occurred at longer evolutionary scales.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Evolution
  • Body Patterning
  • Caenorhabditis elegans / genetics
  • Caenorhabditis elegans / growth & development*
  • Caenorhabditis elegans / metabolism
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / metabolism
  • Female
  • Genes, Homeobox
  • Homeodomain Proteins / genetics
  • Homeodomain Proteins / metabolism
  • Models, Biological
  • Mutation
  • Quantitative Trait Loci
  • Signal Transduction*
  • Vulva / cytology
  • Vulva / growth & development
  • Vulva / metabolism
  • Wnt Proteins / genetics
  • Wnt Proteins / metabolism

Substances

  • Caenorhabditis elegans Proteins
  • Homeodomain Proteins
  • Wnt Proteins