pH neutralization protects against reduction in replicative lifespan following chronological aging in yeast

Cell Cycle. 2012 Aug 15;11(16):3087-96. doi: 10.4161/cc.21465. Epub 2012 Aug 8.


Chronological and replicative aging have been studied in yeast as alternative paradigms for post-mitotic and mitotic aging, respectively. It has been known for more than a decade that cells of the S288C background aged chronologically in rich medium have reduced replicative lifespan relative to chronologically young cells. Here we report replication of this observation in the diploid BY4743 strain background. We further show that the reduction in replicative lifespan from chronological aging is accelerated when cells are chronologically aged under standard conditions in synthetic complete medium rather than rich medium. The loss of replicative potential with chronological age is attenuated by buffering the pH of the chronological aging medium to 6.0, an intervention that we have previously shown can extend chronological lifespan. These data demonstrate that extracellular acidification of the culture medium can cause intracellular damage in the chronologically aging population that is asymmetrically segregated by the mother cell to limit subsequent replicative lifespan.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acids / metabolism
  • Buffers
  • Cell Cycle
  • Culture Media / metabolism
  • DNA Replication*
  • Flow Cytometry
  • Hydrogen-Ion Concentration
  • Microbial Viability*
  • Mitochondria / metabolism
  • Mitochondria / physiology
  • Mitosis
  • Organic Chemicals / metabolism
  • Oxidative Stress*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae / physiology*
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism
  • Staining and Labeling / methods
  • Time Factors


  • Acids
  • Buffers
  • Culture Media
  • Organic Chemicals
  • SYTOX Green
  • Saccharomyces cerevisiae Proteins