With recent spread of infectious diseases, worldwide emergency strategies against them have been made. As one of such efforts we planned to develop an educational program for public health manpower using IT, and produced 4 to 8 minute-long flash animations of the following subjects selected by Education Training Expert Committee: Self-care guideline on H1N1; Instruction for medical hospital; Prevention of hospital acquired infections; Method of sampling & transporting. Demonstrative education using an online education system was conducted targeting 10 doctors working in a rural health center to evaluate the developed animations. They received good marks in the factors of credibility and accuracy, but needed to improve in that of interaction with educatees. Our suggestion is that we use the result of our study as a prototype of education contents on infectious diseases, and modify its contents accordingly when a specific disease breaks out.