This study explored and evaluated the feasibility and safety of laparoscopy with a multichannel port using a wound retractor for large adnexal masses. Seven patients scheduled to undergo single-port or 2-port laparoscopy for large adnexal cysts were enrolled. The cystic mass surface was exposed by inserting the wound retractor through the umbilical port, and cystic fluid was aspirated using a suction-irrigation system after puncture with an Ochsner trocar. Then, laparoscopic adnexal surgery was performed in the usual manner. The mean maximal diameter of cysts, mean operation time, and mean postoperative hospital stay were 17.8 ± 5.4 cm, 81.3 ± 28.2 minutes, and 4.4 ± 2.0 days, respectively. Use of multichannel ports for large adnexal cystic masses seems safe and does not require additional cost. The procedure is also cosmetically effective and highly appreciated by patients, because it results in minimal abdominal scarring.