Platelet aggregation and anti-inflammatory effects of garden pea, Desi chickpea and Kabuli chickpea

Acta Pol Pharm. Jul-Aug 2012;69(4):707-11.

Abstract

Inflammation is the natural body defense mechanism for the removal of injurious agents, necrosed cells and tissues from the body. This study was aimed to evaluate the anti-inflammatory and platelet aggregation effects of three medicinal plants of Pakistan. Methanolic extract of garden pea inhibited arachidonic acid (AA)-induced platelet aggregation (IC50 = 35 microg/mL) and platelet activating factor (PAF)-induced platelet aggregation (IC50 = 38 microg/mL) in a dose dependent fashion. Methanolic extract of Desi chickpea inhibited arachidonic acid (AA) induced platelet aggregation (IC50 value = AA = 46 microg/mL) in dose dependent fashion while was found not active against PAF-induced platelet aggregation. Methanolic extract of Kabuli chickpea was found not active against both arachidonic acid (AA)-induced platelet aggregation and PAF-induced platelet aggregation. The best potential to inhibit in vitro COX-2 activity showed garden pea (Pisum sativum: the synthesis of PGE2 reduced by 92% in comparison with untreated control wells) followed by Desi chickpea (Cicer arietinum var; 87% inhibition) and Kabuli chickpea extracts (Cicer arietinum var: 65% inhibition). All extracts were tested at concentration 20 microg/mL. in COX-2 assay. The results indicate that if the same were happening in vito, Garden pea, Desi chickpea and Kabuli chickpea could be useful as natural antithrombotic anti-inflammatory materials.

Publication types

  • Comparative Study

MeSH terms

  • Arachidonic Acid
  • Cicer* / chemistry
  • Cyclooxygenase 2 / metabolism*
  • Cyclooxygenase 2 Inhibitors / chemistry
  • Cyclooxygenase 2 Inhibitors / isolation & purification
  • Cyclooxygenase 2 Inhibitors / pharmacology*
  • Dinoprostone / metabolism
  • Dose-Response Relationship, Drug
  • Humans
  • Indomethacin / pharmacology
  • Methanol / chemistry
  • Peas* / chemistry
  • Plant Extracts / chemistry
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology*
  • Platelet Activating Factor
  • Platelet Aggregation / drug effects*
  • Platelet Aggregation Inhibitors / chemistry
  • Platelet Aggregation Inhibitors / isolation & purification
  • Platelet Aggregation Inhibitors / pharmacology*
  • Platelet Function Tests
  • Recombinant Proteins / antagonists & inhibitors
  • Recombinant Proteins / metabolism
  • Seeds
  • Solvents / chemistry
  • Time Factors

Substances

  • Cyclooxygenase 2 Inhibitors
  • Plant Extracts
  • Platelet Activating Factor
  • Platelet Aggregation Inhibitors
  • Recombinant Proteins
  • Solvents
  • Arachidonic Acid
  • Cyclooxygenase 2
  • PTGS2 protein, human
  • Dinoprostone
  • Indomethacin
  • Methanol