SARS-CoV Regulates Immune Function-Related Gene Expression in Human Monocytic Cells

Viral Immunol. 2012 Aug;25(4):277-88. doi: 10.1089/vim.2011.0099.

Abstract

Severe acute respiratory syndrome (SARS) is characterized by acute respiratory distress syndrome (ARDS) and pulmonary fibrosis, and monocytes/macrophages are the key players in the pathogenesis of SARS. In this study, we compared the transcriptional profiles of SARS coronavirus (SARS-CoV)-infected monocytic cells against that infected by coronavirus 229E (CoV-229E). Total RNA was extracted from infected DC-SIGN-transfected monocytes (THP-1-DC-SIGN) at 6 and 24 h after infection, and the gene expression was profiled in oligonucleotide-based microarrays. Analysis of immune-related gene expression profiles showed that at 24 h after SARS-CoV infection: (1) IFN-α/β-inducible and cathepsin/proteasome genes were downregulated; (2) hypoxia/hyperoxia-related genes were upregulated; and (3) TLR/TLR-signaling, cytokine/cytokine receptor-related, chemokine/chemokine receptor-related, lysosome-related, MHC/chaperon-related, and fibrosis-related genes were differentially regulated. These results elucidate that SARS-CoV infection regulates immune-related genes in monocytes/macrophages, which may be important to the pathogenesis of SARS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Coronavirus 229E, Human / immunology
  • Coronavirus 229E, Human / physiology
  • Gene Expression Profiling*
  • Gene Expression Regulation / immunology*
  • Gene Regulatory Networks
  • Humans
  • Interleukin-8 / genetics
  • Interleukin-8 / metabolism
  • Monocytes / immunology*
  • Monocytes / metabolism
  • Monocytes / virology
  • Oligonucleotide Array Sequence Analysis
  • Proteasome Endopeptidase Complex / genetics
  • Proteasome Endopeptidase Complex / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • SARS Virus / immunology*
  • SARS Virus / physiology
  • Severe Acute Respiratory Syndrome

Substances

  • Interleukin-8
  • RNA, Messenger
  • Proteasome Endopeptidase Complex