Cannabidiol treatment ameliorates ischemia/reperfusion renal injury in rats

Life Sci. 2012 Sep 17;91(7-8):284-92. doi: 10.1016/j.lfs.2012.07.030. Epub 2012 Aug 1.


Aims: To investigate the protective effect of cannabidiol, the major non-psychotropic Cannabis constituent, against renal ischemia/reperfusion injury in rats.

Main methods: Bilateral renal ischemia was induced for 30 min followed by reperfusion for 24h. Cannabidiol (5mg/kg, i.v.) was given 1h before and 12h following the procedure.

Key findings: Ischemia/reperfusion caused significant elevations of serum creatinine and renal malondialdehyde and nitric oxide levels, associated with a significant decrease in renal reduced glutathione. Cannabidiol significantly attenuated the deterioration in the measured biochemical parameters induced by ischemia/reperfusion. Histopathological examination showed that cannabidiol ameliorated ischemia/reperfusion-induced kidney damage. Immunohistochemical analysis revealed that cannabidiol significantly reduced the expression of inducible nitric oxide synthase, tumor necrosis factor-α, cyclooxygenase-2, nuclear factor-κB, Fas ligand and caspase-3, and increased the expression of survivin in ischemic/reperfused kidney tissue.

Significance: Cannabidiol, via its antioxidant and anti-inflammatory properties, may represent a potential therapeutic option to protect against ischemia/reperfusion renal injury.

MeSH terms

  • Animals
  • Cannabidiol / therapeutic use*
  • Creatinine / blood
  • Immunohistochemistry
  • Kidney / blood supply*
  • Kidney / metabolism
  • Male
  • Malondialdehyde / metabolism
  • Nitric Oxide / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Reperfusion Injury / drug therapy*
  • Reperfusion Injury / prevention & control


  • Cannabidiol
  • Nitric Oxide
  • Malondialdehyde
  • Creatinine