R-loops Cause Replication Impairment and Genome Instability During Meiosis

EMBO Rep. 2012 Oct;13(10):923-9. doi: 10.1038/embor.2012.119. Epub 2012 Aug 10.

Abstract

R-loops are harmful structures with a negative impact on transcription and recombination during mitosis, but no information exists for meiosis. We used Saccharomyces cerevisiae and Caenorhabditis elegans THO mutants as a tool to determine the consequences of R-loops in meiosis. We found that both S. cerevisiae and C. elegans THO mutants show defective meiosis and an impairment of premeiotic replication as well as DNA-damage accumulation. Importantly, RNase H partially suppressed the replication impairment and the DNA-damage accumulation. We conclude that R-loops can form during meiosis causing replication impairment with deleterious results.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caenorhabditis elegans / genetics
  • Caenorhabditis elegans / metabolism
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / metabolism*
  • DNA Damage / genetics
  • DNA Replication / genetics*
  • DNA, Fungal / chemistry
  • DNA, Fungal / metabolism
  • Genomic Instability / genetics*
  • Meiosis / genetics*
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism
  • Ribonuclease H / metabolism
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*

Substances

  • Caenorhabditis elegans Proteins
  • DNA, Fungal
  • HPR1 protein, S cerevisiae
  • Nuclear Proteins
  • Saccharomyces cerevisiae Proteins
  • THO2 protein, S cerevisiae
  • THOC-2 protein, C elegans
  • Transcription Factors
  • Ribonuclease H