A systematic phenotypic screen of F-box genes through a tissue-specific RNAi-based approach in Drosophila

J Genet Genomics. 2012 Aug 20;39(8):397-413. doi: 10.1016/j.jgg.2012.05.009. Epub 2012 Jul 14.


F-box proteins are components of the SCF (SkpA-Cullin 1-F-box) E3 ligase complexes, acting as the specificity-determinants in targeting substrate proteins for ubiquitination and degradation. In humans, at least 22 out of 75 F-box proteins have experimentally documented substrates, whereas in Drosophila 12 F-box proteins have been characterized with known substrates. To systematically investigate the genetic and molecular functions of F-box proteins in Drosophila, we performed a survey of the literature and databases. We identified 45 Drosophila genes that encode proteins containing at least one F-box domain. We collected publically available RNAi lines against these genes and used them in a tissue-specific RNAi-based phenotypic screen. Here, we present our systematic phenotypic dataset from the eye, the wing and the notum. This dataset is the first of its kind and represents a useful resource for future studies of the molecular and genetic functions of F-box genes in Drosophila. Our results show that, as expected, F-box genes in Drosophila have regulatory roles in a diverse array of processes including cell proliferation, cell growth, signal transduction, and cellular and animal survival.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Drosophila / genetics*
  • Drosophila / growth & development
  • Drosophila / metabolism
  • Drosophila Proteins / genetics*
  • Drosophila Proteins / metabolism
  • Eye / growth & development
  • Eye / metabolism
  • F-Box Proteins / genetics*
  • F-Box Proteins / metabolism
  • Molecular Sequence Data
  • Organ Specificity
  • Phenotype
  • RNA Interference*
  • Wings, Animal / growth & development
  • Wings, Animal / metabolism


  • Drosophila Proteins
  • F-Box Proteins