(1)H-NMR-based metabolomic analysis of the effect of moderate wine consumption on subjects with cardiovascular risk factors

Electrophoresis. 2012 Aug;33(15):2345-54. doi: 10.1002/elps.201100646.


Moderate wine consumption is associated with health-promoting activities. An H-NMR-based metabolomic approach was used to identify urinary metabolomic differences of moderate wine intake in the setting of a prospective, randomized, crossover, and controlled trial. Sixty-one male volunteers with high cardiovascular risk factors followed three dietary interventions (28 days): dealcoholized red wine (RWD) (272mL/day, polyphenol control), alcoholized red wine (RWA) (272mL/day) and gin (GIN) (100mL/day, alcohol control). After each period, 24-h urine samples were collected and analyzed by (1) H-NMR. According to the results of a one-way ANOVA, significant markers were grouped in four categories: alcohol-related markers (ethanol); gin-related markers; wine-related markers; and gut microbiota markers (hippurate and 4-hydroxphenylacetic acid). Wine metabolites were classified into two groups; first, metabolites of food metabolome: tartrate (RWA and RWD), ethanol, and mannitol (RWA); and second, biomarkers that relates to endogenous modifications after wine consumption, comprising branched-chain amino acid (BCAA) metabolite (3-methyl-oxovalerate). Additionally, a possible interaction between alcohol and gut-related biomarkers has been identified. To our knowledge, this is the first time that this approach has been applied in a nutritional intervention with red wine. The results show the capacity of this approach to obtain a comprehensive metabolome picture including food metabolome and endogenous biomarkers of moderate wine intake.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohol Drinking / metabolism*
  • Alcohol Drinking / urine
  • Analysis of Variance
  • Biomarkers / metabolism
  • Biomarkers / urine
  • Cardiovascular Diseases / metabolism*
  • Cardiovascular Diseases / urine
  • Cross-Over Studies
  • Ethanol / administration & dosage
  • Ethanol / metabolism
  • Flavonoids / administration & dosage
  • Flavonoids / metabolism
  • Humans
  • Male
  • Metabolome / drug effects*
  • Metabolomics
  • Metagenome
  • Middle Aged
  • Nuclear Magnetic Resonance, Biomolecular / methods*
  • Phenols / administration & dosage
  • Phenols / metabolism
  • Prospective Studies
  • Risk Factors
  • Wine*


  • Biomarkers
  • Flavonoids
  • Phenols
  • Ethanol