Evaluating the toxicity of hydroxyapatite nanoparticles in catfish cells and zebrafish embryos

Small. 2013 May 27;9(9-10):1734-41. doi: 10.1002/smll.201200639. Epub 2012 Aug 9.


The toxicity of needle-(nHA-ND) and rod-shaped (nHA-RD) hydroxyapatite (HA) nanoparticles is evaluated in vitro on catfish B-cells (3B11) and catfish T-cells (28s.3) and in vivo on zebrafish embryos to determine if biological effects are similar to the effects seen in mammalian in vitro systems. Neither nHA-ND nor nHA-RD affect cell viability at concentrations of 10 to 300 μg mL(-1) . However, 30 μg mL(-1) needle-shaped nHA lower metabolic activity of the cells. Axial deformations are seen in zebrafish exposed to 300 μg mL(-1) needle shaped nHA after 120 h. For the first time, nHA is reported to cause zebrafish hatching delay. The lowest concentration (3 μg mL(-1) ) of both types of nHA cause the highest hatching inhibition and needle-shaped nHA exposed zebrafish exhibit the lowest hatch at 72 h post fertilization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Lymphocytes / drug effects*
  • Catfishes*
  • Dose-Response Relationship, Drug
  • Durapatite / toxicity*
  • Embryo, Nonmammalian / drug effects*
  • In Vitro Techniques
  • Microscopy, Electron, Transmission
  • Nanoparticles / toxicity*
  • T-Lymphocytes / drug effects*
  • Zebrafish / embryology*


  • Durapatite