Changes in antioxidant status and cardiovascular risk factors of overweight young men after six weeks supplementation of whey protein isolate and resistance training

Appetite. 2012 Dec;59(3):673-8. doi: 10.1016/j.appet.2012.08.005. Epub 2012 Aug 10.


The study's purpose was to examine the effects of whey protein supplementation and resistance training on antioxidant status and cardiovascular risk factors in overweight young men. Thirty healthy male subjects (age, 23.4±3.6years; body mass index, 25-30kg/m(2)) were randomly divided into three groups of 10 persons including; Experimental group 1: resistance training+whey supplement (RW); Experimental group 2: resistance training+placebo (RP), and Control group (C). Subjects in intervention groups underwent 3 resistance training sessions per week, each session with 60-70% 1RM, for 6weeks. No significant changes in fibrinogen level, fasting blood glucose, resting systolic and diastolic blood pressures, waist to hip ratio (WHR), and body mass index were observed in any of the groups. Total antioxidant capacity (TAC), cholesterol and HDL varied significantly in the RW group compared with the pre-test. We found significant changes in both RW and RP groups for glutathione, vitamin C, LDL, and triglyceride levels. In addition, in the post-test, TAC, glutathione, and HDL levels were higher in the RW in comparison to C group. Research findings showed that although exercise can lead to antioxidant system improvement and reduce some cardiovascular risk factors among overweight subjects, the combination of resistance training and whey consumption is more effective.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Adult
  • Antioxidants / metabolism*
  • Ascorbic Acid / blood
  • Cardiovascular Diseases / blood
  • Cardiovascular Diseases / etiology
  • Cardiovascular Diseases / prevention & control*
  • Cholesterol / blood
  • Cholesterol, HDL / blood
  • Cholesterol, LDL / blood
  • Dietary Proteins / pharmacology
  • Dietary Proteins / therapeutic use*
  • Dietary Supplements
  • Glutathione / blood
  • Humans
  • Lipids / blood*
  • Male
  • Milk Proteins / pharmacology
  • Milk Proteins / therapeutic use*
  • Overweight / blood
  • Overweight / complications
  • Overweight / therapy*
  • Reference Values
  • Resistance Training*
  • Risk Factors
  • Triglycerides / blood
  • Whey Proteins
  • Young Adult


  • Antioxidants
  • Cholesterol, HDL
  • Cholesterol, LDL
  • Dietary Proteins
  • Lipids
  • Milk Proteins
  • Triglycerides
  • Whey Proteins
  • Cholesterol
  • Glutathione
  • Ascorbic Acid