Dual RNA-seq of Pathogen and Host

Nat Rev Microbiol. 2012 Sep;10(9):618-30. doi: 10.1038/nrmicro2852.

Abstract

A comprehensive understanding of host-pathogen interactions requires a knowledge of the associated gene expression changes in both the pathogen and the host. Traditional, probe-dependent approaches using microarrays or reverse transcription PCR typically require the pathogen and host cells to be physically separated before gene expression analysis. However, the development of the probe-independent RNA sequencing (RNA-seq) approach has begun to revolutionize transcriptomics. Here, we assess the feasibility of taking transcriptomics one step further by performing 'dual RNA-seq', in which gene expression changes in both the pathogen and the host are analysed simultaneously.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Bacterial Infections / pathology
  • Computational Biology / methods
  • Disease Models, Animal
  • Gene Expression Profiling / methods*
  • Host-Pathogen Interactions*
  • Humans
  • Mycoses / pathology
  • Plant Diseases / microbiology
  • Protozoan Infections / pathology
  • Sequence Analysis, RNA / methods*