Red blood cell storage in SAGM and AS3: a comparison through the membrane two-dimensional electrophoresis proteome

Blood Transfus. 2012 May;10 Suppl 2(Suppl 2):s46-54. doi: 10.2450/2012.008S.


Background: SAGM is currently the standard additive solution used in Europe, while AS-3 is the third additive solution that has been licensed in the USA, and is also the one used in part of Canada. Although AS-3 is based on a saline-adenine-glucose solution, it also contains citrate and phosphate. Storage of red blood cell concentrates in CPD-SAGM is known to lead to the accumulation of a wide series of storage lesions, including membrane protein fragmentation and vesiculation, as we could previously determine through 2-dimensional gel electrophoresis.

Materials and methods: Through 2D-SDS-IEF-polyacrilamide gel electrophoresis we performed a time course analysis (day 0, 21 and 42 of storage) of red blood cell membranes from leukocyte-filtered concentrates either stored in CPD-SAGM or CP2D-AS-3.

Results and discussion: From the present study it emerges that the membrane protein profile of red blood cells stored in presence of AS-3 appears to be slightly different from (better than) previous reports on SAGM-stored counterparts. However, the increase of total membrane spot number due to the presence of fragments at day 21 and the significant decrease at day 42 are suggestive of a universal phenomenon which is not efficiently tackled by either of the two additive solutions investigated in the present study.

Conclusion: To further delve into the storage lesion issue for RBCs stored in AS-3, it would be interesting in the future to assay metabolic changes over storage progression as well.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenine*
  • Blood Preservation / methods*
  • Citric Acid*
  • Electrophoresis, Gel, Two-Dimensional
  • Erythrocytes*
  • Glucose*
  • Humans
  • Mannitol*
  • Organ Preservation Solutions*
  • Phosphoric Acids*
  • Proteome*
  • Sodium Chloride*


  • AS-3 solution
  • Organ Preservation Solutions
  • Phosphoric Acids
  • Proteome
  • Citric Acid
  • Mannitol
  • Sodium Chloride
  • Glucose
  • Adenine