The potential of a niacinamide dominated cosmeceutical formulation on fibroblast activity and wound healing in vitro

Int Wound J. 2014 Apr;11(2):152-8. doi: 10.1111/j.1742-481X.2012.01052.x. Epub 2012 Aug 14.


Knowledge on the intrinsic mechanisms involved in wound healing provides opportunity for various therapeutic strategies. The manipulation of dermal fibroblast proliferation and differentiation might prove to beneficially augment wound healing. This study evaluated the combined effects of niacinamide, L-carnosine, hesperidin and Biofactor HSP(®) on fibroblast activity. The effects on fibroblast collagen production, cellular proliferation, migration and terminal differentiation were assessed. In addition, the authors determined the effects on in vitro wound healing. The optimal concentrations of actives were determined in vitro. Testing parameters included microscopic morphological cell analysis, cell viability and proliferation determination, calorimetric collagen detection and in vitro wound healing dynamics. Results show that 0·31 mg/ml niacinamide, 0·10 mg/ml L-carnosine, 0·05 mg/ml hesperidin and 5·18 µg/ml Biofactor HSP® proved optimal in vitro. The results show that fibroblast collagen synthesis was increased alongside with cellular migration and proliferation.

Keywords: l-Carnosine; Dermal fibroblasts; Hesperidin; HSP70 homologue; Niacinamide; Wound healing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carnosine / pharmacology
  • Cell Physiological Phenomena / drug effects
  • Cell Physiological Phenomena / physiology
  • Cells, Cultured
  • Collagen / biosynthesis
  • Cosmetics / pharmacology*
  • Dermis / cytology
  • Dermis / metabolism
  • Dose-Response Relationship, Drug
  • Drug Combinations
  • Fibroblasts / drug effects*
  • Fibroblasts / metabolism
  • HSP70 Heat-Shock Proteins / pharmacology
  • Hesperidin / pharmacology
  • Humans
  • Niacinamide / pharmacology*
  • Wound Healing / drug effects*
  • Wound Healing / physiology


  • Cosmetics
  • Drug Combinations
  • HSP70 Heat-Shock Proteins
  • Niacinamide
  • Carnosine
  • Collagen
  • Hesperidin