The prophylactic effect of vitamin C on oxidative stress indexes in rat eyes following exposure to radiofrequency wave generated by a BTS antenna model

Int J Radiat Biol. 2013 Feb;89(2):128-31. doi: 10.3109/09553002.2012.721051. Epub 2012 Sep 7.


Purpose: This study was conducted to evaluate the effect of radiofrequency wave (RFW)-induced oxidative stress in the eye and the prophylactic effect of vitamin C on this organ by measuring the antioxidant enzymes activity including: glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT), and malondialdehyde (MDA).

Materials and methods: Thirty-two adult male Sprague-Dawley rats were randomly divided into four experimental groups and treated daily for 45 days as follows: Control, vitamin C (L-ascorbic acid 200 mg/kg of body weight/day by gavage), test (exposed to 900 MHz RFW) and the treated group (received vitamin C in addition to exposure to RFW). At the end of the experiment all animals were sacrificed, their eyes were removed and were used for measurement of antioxidant enzymes and MDA activity.

Results: The results indicate that exposure to RFW in the test group decreased antioxidant enzymes activity and increased MDA compared with the control groups (P < 0.05). In the treated group vitamin C improved antioxidant enzymes activity and reduced MDA compared to the test group (P < 0.05).

Conclusions: It can be concluded that RFW causes oxidative stress in the eyes and vitamin C improves the antioxidant enzymes activity and decreases MDA.

MeSH terms

  • Animals
  • Antioxidants / metabolism
  • Ascorbic Acid / pharmacology*
  • Catalase / metabolism
  • Eye / drug effects*
  • Eye / metabolism
  • Eye / radiation effects*
  • Glutathione Peroxidase / metabolism
  • Lipid Peroxidation / drug effects
  • Lipid Peroxidation / radiation effects
  • Male
  • Malondialdehyde / metabolism
  • Oxidative Stress / drug effects*
  • Oxidative Stress / radiation effects*
  • Radio Waves / adverse effects*
  • Rats
  • Rats, Sprague-Dawley
  • Superoxide Dismutase / metabolism


  • Antioxidants
  • Malondialdehyde
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Ascorbic Acid