Abstract
Affinity precipitation is a non-chromatographic method which is useful for purification and refolding of proteins. Quite often, a stimuli-sensitive polymer can be identified which selectively binds to the desired protein. For separation, the protein can be recovered from the precipitate of the protein-smart polymer complex. In case of a refolding experiment, binding of the solubilized protein (in its denatured form) with the polymer leads to the refolding of the protein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alginates / chemistry
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Chemical Precipitation
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Chitosan / chemistry
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Glucuronic Acid / chemistry
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Hexuronic Acids / chemistry
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Molecular Imprinting / methods*
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Polymethacrylic Acids / chemistry
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Protein Denaturation
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Protein Refolding*
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Proteins / chemistry
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Proteins / isolation & purification*
Substances
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Alginates
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Hexuronic Acids
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Polymethacrylic Acids
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Proteins
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methylmethacrylate-methacrylic acid copolymer
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Glucuronic Acid
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Chitosan