Decalcifying effect of different ethylenediaminetetraacetic acid irrigating solutions and tetraclean on root canal dentin

J Endod. 2012 Sep;38(9):1239-43. doi: 10.1016/j.joen.2012.06.010. Epub 2012 Jul 10.

Abstract

Introduction: The objective of this study was to evaluate and compare in vitro the decalcifying capability on root canal dentin of 4 different irrigating solutions.

Methods: Twenty-five freshly extracted maxillary central incisors were selected. The canals were prepared to obtain a total of 4 samples from each root. The samples were randomly divided into 5 equal experimental groups (n = 20) according to the irrigating solutions tested (Tetraclean, Largal Ultra, 17% ethylenediaminetetraacetic acid, Tubuliclean). For each irrigating solution, the specimens were immersed for 5, 10, and 15 minutes. After exposures, the concentration of Ca(2+) extracted was measured by inductively coupled plasma-atomic emission spectrometry. Data were analyzed by means of nonparametric tests, and statistical analysis was performed.

Results: The higher release of Ca(2+) was observed in samples treated with Tetraclean; this citric acid-based agent showed significantly higher decalcifying capability. For all irrigating solutions tested, 10 minutes of application are sufficient to obtain the maximum Ca(2+) release.

Conclusions: The results indicated that Tetraclean could be applied to obtain more efficient removal of the smear layer and to facilitate the biomechanical instrumentation procedures.

Publication types

  • Comparative Study
  • Randomized Controlled Trial

MeSH terms

  • Calcium / analysis
  • Cetrimonium
  • Cetrimonium Compounds / pharmacology*
  • Citric Acid / pharmacology*
  • Dental Pulp Cavity / chemistry
  • Dental Pulp Cavity / drug effects*
  • Dentin / chemistry
  • Dentin / drug effects*
  • Doxycycline / pharmacology*
  • Drug Combinations
  • Edetic Acid / pharmacology*
  • Humans
  • Materials Testing
  • Middle Aged
  • Polymers / pharmacology
  • Polypropylenes / pharmacology*
  • Propylene Glycols / pharmacology
  • Root Canal Irrigants / pharmacology*
  • Root Canal Preparation / methods
  • Smear Layer
  • Spectrophotometry, Atomic
  • Time Factors

Substances

  • Cetrimonium Compounds
  • Drug Combinations
  • Polymers
  • Polypropylenes
  • Propylene Glycols
  • Root Canal Irrigants
  • Tetraclean
  • polypropylene glycol
  • Citric Acid
  • Largal Ultra
  • Edetic Acid
  • Doxycycline
  • Calcium
  • Cetrimonium