Rapid automated analysis of glutathione reductase, peroxidase, and S-transferase activity: application to cisplatin-induced toxicity

Clin Biochem. 1990 Dec;23(6):501-4. doi: 10.1016/0009-9120(90)80039-l.


We describe a rapid kinetic method for the automated determination of the xenobiotic-metabolizing enzymes glutathione reductase, glutathione peroxidase and glutathione S-transferase, and its application to the study of cisplatin-induced toxicity. Liver, kidney and urine from control and cisplatin-treated rats were used as the source of enzymes. Advantages over conventional spectrophotometric methods include speed (25 assays in 4 min), small sample size, and improved precision. We show that glutathione S-transferase activity in liver is slightly reduced by cisplatin treatment, whereas all three enzymes are reduced in the kidney. Glutathione-S-transferase activity appeared in urine between the third and seventh days after cisplatin injection. Using these enzyme activities in cisplatin-treated rats, we suggest that the renal enzymes are more sensitive markers of toxicity than hepatic enzymes.

MeSH terms

  • Animals
  • Autoanalysis
  • Cisplatin / toxicity*
  • Clinical Enzyme Tests / methods*
  • Glutathione Peroxidase / analysis*
  • Glutathione Reductase / analysis*
  • Glutathione Transferase / analysis*
  • Glutathione Transferase / urine
  • Kidney / enzymology
  • Liver / enzymology
  • Male
  • Rats
  • Rats, Inbred BN
  • Reproducibility of Results
  • Spectrophotometry / instrumentation


  • Glutathione Peroxidase
  • Glutathione Reductase
  • Glutathione Transferase
  • Cisplatin