Image-based analysis of light-grown seedling hypocotyls in Arabidopsis

Methods Mol Biol. 2012;918:1-7. doi: 10.1007/978-1-61779-995-2_1.


Time-resolved hypocotyl length measurements in seedlings have the potential to greatly aid genetic studies looking at light, hormone, and circadian regulation of cell expansion. Recently, several computer-based tools have been developed to quantify hypocotyl length during photomorphogenesis and early seedling development. Here we detail a method for quantifying Arabidopsis seedling hypocotyls in an image-based assay, focusing on light-grown seedlings responding to shade conditions.

MeSH terms

  • Arabidopsis / growth & development*
  • Arabidopsis / radiation effects*
  • Hypocotyl / growth & development*
  • Hypocotyl / radiation effects*
  • Image Processing, Computer-Assisted
  • Light*
  • Molecular Imaging / methods*
  • Time Factors