Disease resistance to Pectobacterium carotovorum is negatively modulated by the Arabidopsis Lectin Receptor Kinase LecRK-V.5

Plant Signal Behav. 2012 Sep 1;7(9):1070-2. doi: 10.4161/psb.21013. Epub 2012 Aug 17.

Abstract

Plant stomata function in disease resistance by restricting bacteria entry inside leaves. During plant-bacteria interactions, stomatal closure is initiated by the recognition of Microbe-Associated Molecular Patterns (MAMPs). Recently, we have shown that the Lectin Receptor Kinase V.5 (LecRK-V.5) negatively regulates bacterium- and MAMP-induced stomatal closure upstream of Reactive Oxygen Species (ROS) production mediated by abscisic acid signaling. Closed stomata in lecrk-V.5 mutants are correlated with constitutive high level of ROS in guard cells. Consequently, lecrk-V.5 mutants are more resistant to hemi-biotrophic pathogen Pseudomonas syringae pv tomato DC3000 (Pst DC3000). In this report, we further investigate the role of LecRK-V.5 in resistance against necrotrophic bacteria Pectobacterium carotovorum ssp. carotovorum (Pcc). Upon surface-inoculation lecrk-V.5 mutants exhibited enhanced resistance against Pcc whereas a wild-type level of resistance was observed using infiltration-inoculation, an inoculation method that bypasses the epidermal barrier. Enhanced resistance of dip-inoculated lecrk-V.5 mutants against necrotrophic bacteria, that induce different defense responses than hemi-biotrophic bacteria, further suggests a possible role for LecRK-V.5 in stomatal immunity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abscisic Acid / metabolism
  • Arabidopsis / enzymology
  • Arabidopsis / genetics*
  • Arabidopsis / microbiology
  • Arabidopsis Proteins / genetics*
  • Arabidopsis Proteins / metabolism
  • Disease Resistance / genetics*
  • Disease Resistance / physiology
  • Genes, Plant*
  • Mutation
  • Pectobacterium carotovorum*
  • Plant Diseases / genetics
  • Plant Diseases / microbiology*
  • Plant Epidermis / physiology
  • Plant Leaves / physiology
  • Plant Stomata / physiology*
  • Protein-Serine-Threonine Kinases / genetics*
  • Protein-Serine-Threonine Kinases / metabolism
  • Reactive Oxygen Species / metabolism
  • Signal Transduction

Substances

  • Arabidopsis Proteins
  • Reactive Oxygen Species
  • Abscisic Acid
  • ATHLECRK protein, Arabidopsis
  • Protein-Serine-Threonine Kinases