Effects of penta-O-galloyl-β-D-glucose on human neutrophil function: significant down-regulation of L-selectin expression

Phytother Res. 2013 Jul;27(7):986-92. doi: 10.1002/ptr.4822. Epub 2012 Aug 16.

Abstract

Penta-O-galloyl-β-D-glucose (PGG) occurrs in high concentrations in medicinal herbs such as Rhus chinensis, Paeonia suffruticosa, Acer truncatum and Terminalia chebula, which demonstrate anti-inflammatory activity. We investigated the effect of PGG on stimulated and non-stimulated neutrophils in processes which included reactive oxygen species generation (ROS), metalloproteinase-9 and interleukin-8 secretion (IL-8), β₂ integrin (CD11b) and L-selectin (CD62L) expression and apoptosis. In concentrations of 5 μM-20 μM, PGG demonstrated statistically significant inhibition of ROS generation, IL-8 secretion and β₂ integrin expression in stimulated neutrophils. The inhibition of L-selectin expression by PGG resulted in prevention in neutrophils' endothelial attachment. The result obtained may explain the anti-inflammatory activity of this compound and underline the contribution of PGG in the activity of PGG rich plant extracts.

Keywords: L-selectin; inflammation; neutrophils; penta-O-galloyl-β-D-glucosel.

MeSH terms

  • Acer / chemistry
  • Anti-Inflammatory Agents / pharmacology*
  • Apoptosis / drug effects*
  • CD11b Antigen / metabolism
  • Down-Regulation / drug effects
  • Glucose / pharmacology
  • Humans
  • Hydrolyzable Tannins / pharmacology*
  • Interleukin-8 / biosynthesis*
  • L-Selectin / metabolism*
  • Matrix Metalloproteinase 9 / metabolism
  • Neutrophils / drug effects*
  • Neutrophils / metabolism
  • Paeonia / chemistry
  • Plant Extracts / pharmacology*
  • Reactive Oxygen Species / metabolism
  • Rhus / chemistry
  • Terminalia / chemistry

Substances

  • Anti-Inflammatory Agents
  • CD11b Antigen
  • Hydrolyzable Tannins
  • ITGAM protein, human
  • Interleukin-8
  • Plant Extracts
  • Reactive Oxygen Species
  • L-Selectin
  • beta-penta-O-galloyl-glucose
  • Matrix Metalloproteinase 9
  • Glucose