Cerebrospinal fluid proteome of patients with acute Lyme disease

J Proteome Res. 2012 Oct 5;11(10):4814-22. doi: 10.1021/pr300577p. Epub 2012 Aug 31.


During acute Lyme disease, bacteria can disseminate to the central nervous system (CNS), leading to the development of meningitis and other neurologic symptoms. Here we have analyzed pooled cerebrospinal fluid (CSF) allowing a deep view into the proteome for patients diagnosed with early disseminated Lyme disease and CSF inflammation. Additionally, we analyzed individual patient samples and quantified differences in protein abundance employing label-free quantitative mass spectrometry-based methods. We identified 108 proteins that differ significantly in abundance in patients with acute Lyme disease from controls. Comparison between infected patients and control subjects revealed differences in proteins in the CSF associated with cell death localized to brain synapses and others that likely originate from brain parenchyma.

MeSH terms

  • Acute Disease
  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Analysis of Variance
  • Area Under Curve
  • Case-Control Studies
  • Child
  • Child, Preschool
  • Female
  • Humans
  • Limit of Detection
  • Lyme Neuroborreliosis / cerebrospinal fluid*
  • Male
  • Metabolic Networks and Pathways
  • Middle Aged
  • Nerve Tissue Proteins / cerebrospinal fluid
  • Proteome / metabolism*
  • ROC Curve
  • Young Adult


  • Nerve Tissue Proteins
  • Proteome