Mouse ooplasm confers context-specific reprogramming capacity

Nat Genet. 2012 Sep;44(9):978-80. doi: 10.1038/ng.2382. Epub 2012 Aug 19.


Enucleated oocytes have the distinctive ability to reprogram somatic nuclei back to totipotency. Here, we investigate genome-scale DNA methylation patterns after nuclear transfer and compare them to the dynamics at fertilization. We identify specific targets for DNA demethylation after nuclear transfer, such as germline-associated promoters, as well as unique limitations that include certain repetitive element classes.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cytoplasm / physiology*
  • DNA Methylation / physiology
  • Embryo, Mammalian
  • Embryonic Development* / physiology
  • Female
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred DBA
  • Nuclear Transfer Techniques
  • Oocytes / cytology*
  • Organ Specificity
  • Promoter Regions, Genetic / genetics
  • Promoter Regions, Genetic / physiology
  • Sperm-Ovum Interactions / physiology

Associated data

  • GEO/GSE38711