A versatile assay for monitoring in vivo-like transendothelial migration of neutrophils

Lab Chip. 2012 Oct 21;12(20):3861-5. doi: 10.1039/c2lc40445a.


Spatiotemporal analysis of the inflammatory response has been limited by the difficulties of in vivo imaging and reconstitution of inflammation in vitro. Here, we present a novel method for establishing in vivo-like inflammatory models in a microfluidic device and quantitatively measuring the three-dimensional transmigration of neutrophils during the inflammatory process. This enabled us to concurrently characterize transendothelial migration behaviors of neutrophils under the influence of various inflammatory stimuli.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Humans
  • Inflammation / metabolism
  • Microfluidic Analytical Techniques / instrumentation*
  • Microfluidic Analytical Techniques / methods*
  • Models, Biological*
  • Neutrophils / cytology*
  • Neutrophils / metabolism*
  • Transendothelial and Transepithelial Migration*