Selective effects of hydroxyapatite nanoparticles on osteosarcoma cells and osteoblasts

J Mater Sci Mater Med. 2012 Sep;23(9):2245-51. doi: 10.1007/s10856-012-4703-6. Epub 2012 Aug 19.


The effects of hydroxyapatite nanoparticles (HA-NPs) on two kinds of cells, human MG63 cells and the normal osteoblasts were investigated. According to the MTT assay and fluorescent staining assay, it was proved that HA-NPs could inhibit the growth of MG63 cells but slightly support proliferation of the osteoblasts. Meanwhile, transmission electron microscopy (TEM) was employed to observe the ultrastructural alterations of both cells. The TEM results showed that HA-NPs had entered the two kinds of cells. Typical apoptosis was observed in the MG63 cells, especially in the group of 250 μg/mL with 5 days culture. While no apoptosis could be found in the normal osteoblasts at any concentration group of HA-NPs. Our results suggested that the HA-NPs had selective effects to different kinds of cells: supporting proliferation to the normal bone cells while causing apoptosis to the osteosarcoma cells.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bone Neoplasms / pathology*
  • Bone Neoplasms / ultrastructure
  • Cell Survival / drug effects
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Durapatite / chemistry
  • Durapatite / pharmacology*
  • Humans
  • Microscopy, Electron, Transmission
  • Nanoparticles / chemistry
  • Organ Specificity / drug effects
  • Organelles / drug effects
  • Organelles / physiology
  • Organelles / ultrastructure
  • Osteoblasts / drug effects*
  • Osteoblasts / physiology
  • Osteoblasts / ultrastructure
  • Osteosarcoma / pathology*
  • Osteosarcoma / ultrastructure


  • Durapatite