The paper deals with the identification results of mycobacteria isolated from man, animals and environmental objects. Mycobacterium bovis had a high resistance towards all trial drugs. Nevertheless, the representatives of atypical mycobacteria were resistant to streptomycin, isoniazid and PAS. The analysis of a specific spectrum of mycobacteria isolated from the environmental objects indicated that, according to Ranyon, it contained representatives of different groups. Under unfavorable tuberculosis epidemiologic and epizootologic conditions, the study of mycobacteria circulating on the animal habitat objects is of great practical importance.