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, 7 (7), e41176

Evaluation of a Single Procedure Allowing the Isolation of Enteropathogenic Yersinia Along With Other Bacterial Enteropathogens From Human Stools


Evaluation of a Single Procedure Allowing the Isolation of Enteropathogenic Yersinia Along With Other Bacterial Enteropathogens From Human Stools

Cyril Savin et al. PLoS One.


Enteropathogenic Yersinia are among the most frequent agents of human diarrhea in temperate and cold countries. However, the incidence of yersiniosis is largely underestimated because of the peculiar growth characteristics of pathogenic Yersinia, which make their isolation from poly-contaminated samples difficult. The use of specific procedures for Yersinia isolation is required, but is expensive and time consuming, and therefore is not systematically performed in clinical pathology laboratories. A means to circumvent this problem would be to use a single procedure for the isolation of all bacterial enteropathogens. Since the Statens Serum Institut enteric medium (SSI) has been reported to allow the growth at 37°C of most gram-negative bacteria, including Yersinia, our study aimed at evaluating its performances for Yersinia isolation, as compared to the commonly used Yersinia-specific semi-selective Cefsulodin-Irgasan-Novobiocin medium (CIN) incubated at 28°C. Our results show that Yersinia pseudotuberculosis growth was strongly inhibited on SSI at 37°C, and therefore that this medium is not suitable for the isolation of this species. All Yersinia enterocolitica strains tested grew on SSI, while some non-pathogenic Yersinia species were inhibited. The morphology of Y. enterocolitica colonies on SSI allowed their differentiation from various other gram-negative bacteria commonly isolated from stool samples. However, in artificially contaminated human stools, the recovery of Y. enterocolitica colonies on SSI at 37°C was difficult and was 3 logs less sensitive than on CIN at 28°C. Therefore, despite its limitations, the use of a specific procedure (CIN incubated at 28°C) is still required for an efficient isolation of enteropathogenic Yersinia from stools.

Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.


Figure 1
Figure 1. Colonies of Y. enterocolitica alone or with enterobacteria after 24 h of growth on SSI at 28°C and 37°C.
Y. enterocolitica 4/O:3 (strain IP29492) alone (A), or mixed with K. oxytoca (B), E. coli. (C), C. sakazakii (D). Colonies of Y. enterocolitica are indicated with a star on mixed plates. The black bar represents 2 mm.

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