A RAD tag derived marker based eggplant linkage map and the location of QTLs determining anthocyanin pigmentation

PLoS One. 2012;7(8):e43740. doi: 10.1371/journal.pone.0043740. Epub 2012 Aug 17.

Abstract

Both inter- and intra-specific maps have been developed in eggplant (Solanum melongena L.). The former benefit from an enhanced frequency of marker polymorphism, but their relevance to marker-assisted crop breeding is limited. Combining the restriction-site associated DNA strategy with high throughput sequencing has facilitated the discovery of a large number of functional single nucleotide polymorphism (SNP) markers discriminating between the two eggplant mapping population parental lines '305E40' and '67/3'. A set of 347 de novo SNPs, together with 84 anchoring markers, were applied to the F(2) mapping population bred from the cross '305E40' x '67/3' to construct a linkage map. In all, 415 of the 431 markers were assembled into twelve major and one minor linkage group, spanning 1,390 cM, and the inclusion of established markers allowed each linkage group to be assigned to one of the 12 eggplant chromosomes. The map was then used to discover the genetic basis of seven traits associated with anthocyanin content. Each of the traits proved to be controlled by between one and six quantitative trait loci (QTL), of which at least one was a major QTL. Exploitation of syntenic relationships between the eggplant and tomato genomes facilitated the identification of potential candidate genes for the eggplant QTLs related to anthocyanin accumulation. The intra-specific linkage map should have utility for elucidating the genetic basis of other phenotypic traits in eggplant.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anthocyanins / biosynthesis*
  • Chromosome Mapping / methods*
  • Chromosomes, Plant / genetics
  • Genes, Plant / genetics
  • Genetic Markers / genetics
  • Lycopersicon esculentum / genetics
  • Lycopersicon esculentum / metabolism
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Polymorphism, Single Nucleotide
  • Quantitative Trait Loci / genetics*
  • Solanum melongena / genetics*
  • Solanum melongena / metabolism
  • Synteny

Substances

  • Anthocyanins
  • Genetic Markers
  • Plant Proteins

Grant support

This research was partially supported by the Italian Ministry of Agricultural Alimentary and Forest Politics in the framework of “PROM”, “ESPLORA” and “AGRONANOTECH” projects. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.