Polymorphisms in HIF-1alpha Affect Presence of Lymph Node Metastasis and Can Influence Tumor Size in Squamous-Cell Carcinoma of the Glottic Larynx

Clin Transl Oncol. 2013 May;15(5):358-63. doi: 10.1007/s12094-012-0930-z. Epub 2012 Aug 23.


Background: HIF-1alpha plays a key role in the development and progression of cancer. Its polymorphic variants C1772T and G1790A have been associated with greater susceptibility to cancer and increased tumor progression.

Methods: We determined the distribution of these polymorphisms among 121 patients with glottic cancer and 154 healthy volunteers by PCR-RFLP. We also analyzed the relationship between the presence of these polymorphisms and various clinicopathologic variables.

Results: Advanced tumors (T3-T4) were associated with the TT variant (p = 0.036), which was present in 75 % of T4 tumors (p = 0.008). Among patients with nodal metastasis (N+), 41.7 and 22 % were carrying the TT and GA variants, respectively, compared with 9.4 and 2 % of the patients with no metastasis (N0), (p = 0.006 and p = 0.032).

Conclusions: The presence of the TT and GA variants were associated with lymph node metastasis, while the presence of the TT variant can be associated with larger tumor size.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Carcinoma, Squamous Cell / genetics*
  • Carcinoma, Squamous Cell / pathology
  • Female
  • Genotype
  • Glottis / pathology*
  • Humans
  • Hypoxia-Inducible Factor 1, alpha Subunit / genetics*
  • Laryngeal Neoplasms / genetics*
  • Laryngeal Neoplasms / pathology
  • Lymphatic Metastasis
  • Male
  • Middle Aged
  • Polymorphism, Genetic*


  • Hypoxia-Inducible Factor 1, alpha Subunit