Research resource: RNA-Seq reveals unique features of the pancreatic β-cell transcriptome

Mol Endocrinol. 2012 Oct;26(10):1783-92. doi: 10.1210/me.2012-1176. Epub 2012 Aug 21.


The pancreatic β-cell is critical for the maintenance of glycemic control. Knowing the compendium of genes expressed in β-cells will further our understanding of this critical cell type and may allow the identification of future antidiabetes drug targets. Here, we report the use of next-generation sequencing to obtain nearly 1 billion reads from the polyadenylated RNA of islets and purified β-cells from mice. These data reveal novel examples of β-cell-specific splicing events, promoter usage, and over 1000 long intergenic noncoding RNA expressed in mouse β-cells. Many of these long intergenic noncoding RNA are β-cell specific, and we hypothesize that this large set of novel RNA may play important roles in β-cell function. Our data demonstrate unique features of the β-cell transcriptome.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Female
  • Gene Expression Profiling
  • High-Throughput Nucleotide Sequencing*
  • Insulin-Secreting Cells / metabolism*
  • Male
  • Mice
  • Mice, Transgenic
  • Organ Specificity
  • Promoter Regions, Genetic
  • RNA Splicing
  • RNA, Long Noncoding / genetics
  • RNA, Long Noncoding / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Sequence Analysis, RNA
  • Transcriptome*
  • Tryptophan Hydroxylase / genetics
  • Tryptophan Hydroxylase / metabolism


  • RNA, Long Noncoding
  • RNA, Messenger
  • Tph1 protein, mouse
  • Tryptophan Hydroxylase