Quick regulation of mRNA functions by a few seconds of photoirradiation

Org Biomol Chem. 2012 Oct 14;10(38):7820-5. doi: 10.1039/c2ob25883h.


3-Cyanovinylcarbazole nucleoside, which effectively photocrosslinks to the pyrimidine base in complementary RNA strands, was incorporated into antisense oligonucleotides, and we evaluated the photoreactivity and the sequence selectivity to mutated K-ras oligoRNAs, as well as the regulation of the function of K-ras mRNA. We demonstrated that the reverse transcription and the translation activity of K-ras mRNA were quickly suppressed by a few seconds of photoirradiation with the addition of the photoresponsive antisense ODN.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbazoles / chemistry
  • Cross-Linking Reagents / chemistry
  • Humans
  • Oligonucleotides / chemical synthesis
  • Oligonucleotides / chemistry
  • Oligonucleotides / genetics
  • Photochemical Processes
  • Proto-Oncogene Proteins p21(ras) / chemistry
  • Proto-Oncogene Proteins p21(ras) / genetics
  • Proto-Oncogene Proteins p21(ras) / metabolism
  • Pyrimidines / chemistry
  • RNA, Messenger / chemistry
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism*
  • Real-Time Polymerase Chain Reaction
  • Tumor Cells, Cultured
  • Ultraviolet Rays*


  • Carbazoles
  • Cross-Linking Reagents
  • Oligonucleotides
  • Pyrimidines
  • RNA, Messenger
  • Proto-Oncogene Proteins p21(ras)
  • pyrimidine