PEGylated PRINT nanoparticles: the impact of PEG density on protein binding, macrophage association, biodistribution, and pharmacokinetics

Nano Lett. 2012 Oct 10;12(10):5304-10. doi: 10.1021/nl302638g. Epub 2012 Sep 5.


In this account, we varied PEGylation density on the surface of hydrogel PRINT nanoparticles and systematically observed the effects on protein adsorption, macrophage uptake, and circulation time. Interestingly, the density of PEGylation necessary to promote a long-circulating particle was dramatically less than what has been previously reported. Overall, our methodology provides a rapid screening technique to predict particle behavior in vivo and our results deliver further insight to what PEG density is necessary to facilitate long-circulation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adsorption
  • Animals
  • Female
  • Hydrogels
  • Macrophages / physiology
  • Mice
  • Mice, Inbred BALB C
  • Molecular Conformation
  • Nanoparticles / administration & dosage
  • Nanoparticles / chemistry*
  • Nanotechnology
  • Phagocytosis
  • Polyethylene Glycols / chemistry*
  • Polyethylene Glycols / pharmacokinetics
  • Protein Binding
  • Surface Properties
  • Tissue Distribution


  • Hydrogels
  • Polyethylene Glycols