Kraft lignin biodegradation by Novosphingobium sp. B-7 and analysis of the degradation process

Bioresour Technol. 2012 Nov;123:682-5. doi: 10.1016/j.biortech.2012.07.028. Epub 2012 Jul 20.

Abstract

This study focused on the biodegradation of kraft lignin (KL) by Novosphingobium sp. B-7 using KL as sole carbon source. Results revealed that Novosphingobium sp. B-7 reduced the chemical oxygen demand (COD) by 34.7% in KL mineral salt medium after 7days of incubation. Additionally, the maximum activities of manganese peroxidase (MnP) of 3229.8Ul(-1) and laccase (Lac) of 1275Ul(-1) were observed at 4th and 5th day, respectively. GC-MS analysis indicated that after incubated with Novosphingobium sp. B-7, low molecular weight alcohols and lignin-related monomer compounds such as ethanediol, p-hydroxy benzoic acid and vanillic acid were formed in the system, which strongly confirmed the degradation of KL by Novosphingobium sp. B-7.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biodegradation, Environmental
  • Biological Oxygen Demand Analysis
  • Laccase / metabolism
  • Lignin / metabolism*
  • Peroxidases / metabolism
  • Sphingomonadaceae / growth & development
  • Sphingomonadaceae / metabolism*

Substances

  • Kraft lignin
  • Lignin
  • Laccase
  • Peroxidases
  • manganese peroxidase