A robust plate assay for detection of extracellular microbial protease activity in metagenomic screens and pure cultures

J Microbiol Methods. 2012 Oct;91(1):144-6. doi: 10.1016/j.mimet.2012.08.006. Epub 2012 Aug 19.

Abstract

A robust, efficient and cost-effective agar that utilises lactose free milk powder for identification of bacterial protease activity in pure cultures and metagenomic screens has been developed and tested on protease positive bacteria, selected strains and false protease positives isolated from a previously constructed metagenomic library.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agar
  • Animals
  • Bacteria / enzymology*
  • Bacteria / growth & development
  • Culture Media / chemistry*
  • Milk / metabolism
  • Peptide Hydrolases / analysis*

Substances

  • Culture Media
  • Agar
  • Peptide Hydrolases