Enterohaemorrhagic Escherichia coli exploits a tryptophan switch to hijack host f-actin assembly

Structure. 2012 Oct 10;20(10):1692-703. doi: 10.1016/j.str.2012.07.015. Epub 2012 Aug 23.

Abstract

Intrinsically disordered protein (IDP)-mediated interactions are often characterized by low affinity but high specificity. These traits are essential in signaling and regulation that require reversibility. Enterohaemorrhagic Escherichia coli (EHEC) exploit this situation by commandeering host cytoskeletal signaling to stimulate actin assembly beneath bound bacteria, generating "pedestals" that promote intestinal colonization. EHEC translocates two proteins, EspF(U) and Tir, which form a complex with the host protein IRTKS. The interaction of this complex with N-WASP triggers localized actin polymerization. We show that EspF(U) is an IDP that contains a transiently α-helical N-terminus and dynamic C-terminus. Our structure shows that single EspF(U) repeat forms a high-affinity trimolecular complex with N-WASP and IRTKS. We demonstrate that bacterial and cellular ligands interact with IRTKS SH3 in a similar fashion, but the bacterial protein has evolved to outcompete cellular targets by utilizing a tryptophan switch that offers superior binding affinity enabling EHEC-induced pedestal formation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / chemistry*
  • Amino Acid Sequence
  • Carrier Proteins / chemistry*
  • Conserved Sequence
  • Enterohemorrhagic Escherichia coli / physiology*
  • Escherichia coli Proteins / chemistry*
  • HEK293 Cells
  • Host-Pathogen Interactions*
  • Humans
  • Hydrophobic and Hydrophilic Interactions
  • Intracellular Signaling Peptides and Proteins
  • Microfilament Proteins / chemistry
  • Models, Molecular
  • Molecular Sequence Data
  • Nuclear Magnetic Resonance, Biomolecular
  • Peptide Fragments / chemistry
  • Protein Binding
  • Protein Structure, Quaternary
  • Protein Transport
  • Thermodynamics
  • Tryptophan / chemistry*
  • Wiskott-Aldrich Syndrome Protein, Neuronal / chemistry
  • src Homology Domains

Substances

  • Actins
  • Carrier Proteins
  • Escherichia coli Proteins
  • EspFU protein, E coli
  • IRTKS protein, human
  • Intracellular Signaling Peptides and Proteins
  • Microfilament Proteins
  • Peptide Fragments
  • WASL protein, human
  • Wiskott-Aldrich Syndrome Protein, Neuronal
  • Tryptophan

Associated data

  • PDB/2LNH