A multiagent filovirus DNA vaccine delivered by intramuscular electroporation completely protects mice from ebola and Marburg virus challenge

Hum Vaccin Immunother. 2012 Nov 1;8(11):1703-6. doi: 10.4161/hv.21873. Epub 2012 Aug 24.


We evaluated the immunogenicity and protective efficacy of DNA vaccines expressing the codon-optimized envelope glycoprotein genes of Zaire ebolavirus, Sudan ebolavirus, and Marburg marburgvirus (Musoke and Ravn). Intramuscular or intradermal delivery of the vaccines in BALB/c mice was performed using the TriGrid™ electroporation device. Mice that received DNA vaccines against the individual viruses developed robust glycoprotein-specific antibody titers as determined by ELISA and survived lethal viral challenge with no display of clinical signs of infection. Survival curve analysis revealed there was a statistically significant increase in survival compared to the control groups for both the Ebola and Ravn virus challenges. These data suggest that further analysis of the immune responses generated in the mice and additional protection studies in nonhuman primates are warranted.

Keywords: DNA vaccine; codon-optimized; ebola virus; electroporation; filovirus; marburg virus; mice.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Ebolavirus / immunology
  • Ebolavirus / pathogenicity
  • Electroporation / methods*
  • Female
  • Hemorrhagic Fever, Ebola / immunology
  • Hemorrhagic Fever, Ebola / prevention & control*
  • Marburg Virus Disease / immunology
  • Marburg Virus Disease / prevention & control*
  • Marburgvirus / immunology
  • Marburgvirus / pathogenicity
  • Mice
  • Mice, Inbred BALB C
  • Muscles / metabolism*
  • Vaccines, DNA / administration & dosage*
  • Vaccines, DNA / therapeutic use*


  • Vaccines, DNA