Flavobacterium cheonhonense sp. nov., isolated from a freshwater reservoir

J Microbiol. 2012 Aug;50(4):562-6. doi: 10.1007/s12275-012-1229-z. Epub 2012 Jul 21.


A novel bacterium, designated strain ARSA-15(T), was isolated from a freshwater sample collected from the Cheonho reservoir, Cheonan, Republic of Korea. The isolate was deep-yellow pigment, Gram-negative, rod-shaped, non-motile, and catalase- and oxidase-positive. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belongs to the genus Flavobacterium, and shared less than 97% sequence similarity with recognized Flavobacterium species. The novel species was able to grow at 10-37°C, pH 6.5-10.0, and in 0-0.5% (w/v) NaCl concentrations. Chemotaxonomically, iso-C(15:1), iso-C(15:0), and iso-C(16:0) were observed to be the predominant cellular fatty acid, and menaquinone-6 (MK-6) was the predominant respiratory quinone. The major polar lipid patterns of strain ARSA-19(T) was phosphatidylethanolamine, unknown aminolipid (AL1 and AL2), and unidentified polar lipids (L1, L2, and L3). The genomic DNA G+C content of the isolate was 39.2 mol%. On the basis of polyphasic approach, strain ARSA-15(T) represents a novel species of the genus Flavobacterium, for which the name Flavobacterium cheonhonense sp. nov. is proposed. The type strain is ARSA-15(T) (=KACC 14967(T) =KCTC 23180(T) =JCM 17064(T)).

MeSH terms

  • Base Composition
  • Catalase / metabolism
  • Cluster Analysis
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Fatty Acids / analysis
  • Flavobacterium / classification*
  • Flavobacterium / genetics
  • Flavobacterium / isolation & purification*
  • Flavobacterium / physiology
  • Fresh Water / microbiology*
  • Hydrogen-Ion Concentration
  • Molecular Sequence Data
  • Oxidoreductases / metabolism
  • Phospholipids / analysis
  • Phylogeny
  • Pigments, Biological / metabolism
  • Quinones / analysis
  • RNA, Ribosomal, 16S / genetics
  • Republic of Korea
  • Sequence Analysis, DNA
  • Sodium Chloride / metabolism
  • Temperature


  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Phospholipids
  • Pigments, Biological
  • Quinones
  • RNA, Ribosomal, 16S
  • Sodium Chloride
  • Oxidoreductases
  • Catalase

Associated data

  • GENBANK/GU295972