Complement factors are secreted in human follicular fluid by granulosa cells and are possible oocyte maturation factors

J Obstet Gynaecol Res. 2013 Feb;39(2):522-7. doi: 10.1111/j.1447-0756.2012.01985.x. Epub 2012 Aug 26.


Aims: In this study, we identify components of the complement system present in human follicular fluid that affect oocyte development and maturation.

Material and methods: Using bottom-up liquid chromatography/mass spectrometry/mass spectrometry, we identified complement factors as consistently present in human follicular fluid from 15 different subjects.

Results: According to our gene-chip data, these complement factors are actively produced by granulosa cells.

Conclusions: By applying the computational Ingenuity Pathway Analysis software and database we have identified complement pathways that play a role in oocyte maturation and follicular development.

MeSH terms

  • Adolescent
  • Adult
  • Complement System Proteins / biosynthesis
  • Complement System Proteins / genetics
  • Complement System Proteins / metabolism*
  • Female
  • Follicular Fluid / metabolism*
  • Gene Expression
  • Granulosa Cells / metabolism*
  • Humans
  • Oogenesis*
  • Ovarian Follicle / cytology
  • Ovarian Follicle / diagnostic imaging
  • Ovarian Follicle / growth & development*
  • Ovarian Follicle / metabolism
  • Ultrasonography
  • Young Adult


  • Complement System Proteins