Fluorescent properties and resonance energy transfer of 3,4-bis(2,4-difluorophenyl)-maleimide

Org Biomol Chem. 2012 Oct 14;10(38):7840-6. doi: 10.1039/c2ob25981h.


A fluorescent compound 3,4-bis(2,4-difluorophenyl)-maleimide from the 3,4-diaryl-substituted maleimides was synthesized and determined to have a Stokes shift of 140 nm (λ(abs) 341 nm, λ(em) 481 nm), a high fluorescent quantum yield (Φ(fl) 0.61) and an extinction coefficient ε((340)) of 48 400 M(-1) cm(-1) in dichloromethane. For the first time we demonstrated the successful implementation of a 3,4-diaryl-substituted maleimide molecule as a donor component in FRET experiments.

MeSH terms

  • Crystallography, X-Ray
  • Fluorescence Resonance Energy Transfer*
  • Fluorescence*
  • Hydrogen-Ion Concentration
  • Maleimides / chemical synthesis
  • Maleimides / chemistry*
  • Models, Molecular
  • Molecular Structure


  • 3,4-bis(2,4-difluorophenyl)-maleimide
  • Maleimides