Cloning of two hypothalamic cDNAs encoding tissue-specific transcripts in the preoptic area and testis

Mol Endocrinol. 1990 Aug;4(8):1205-10. doi: 10.1210/mend-4-8-1205.


Neuropeptides are central to the regulation of normal sexual development and reproduction. Two new hypothalamic cDNAs have been identified by Northern blot analysis and molecular cloning. Each potentially encodes a precursor for a unique GnRH-like decapeptide. Northern blot analysis reveals tissue-specific transcripts from each gene in the hypothalamus and testis. These preoptic area regulatory factor genes, PORF-1 and PORF-2, may thus encode members of a family of GnRH-related peptides which have been described in these tissues.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular*
  • DNA / genetics*
  • DNA / isolation & purification
  • Gonadotropin-Releasing Hormone / genetics*
  • Hypothalamus / chemistry*
  • Male
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Preoptic Area / chemistry*
  • RNA, Messenger / analysis
  • Rats
  • Rats, Inbred Strains
  • Regulatory Sequences, Nucleic Acid
  • Testis / chemistry*
  • Transcription, Genetic


  • RNA, Messenger
  • Gonadotropin-Releasing Hormone
  • DNA

Associated data

  • GENBANK/X13191
  • GENBANK/X13192
  • GENBANK/X53231
  • GENBANK/X53232