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. 2012 Jul;9(7):671-5.
doi: 10.1038/nmeth.2089.

NIH Image to ImageJ: 25 Years of Image Analysis

Free PMC article

NIH Image to ImageJ: 25 Years of Image Analysis

Caroline A Schneider et al. Nat Methods. .
Free PMC article


For the past 25 years NIH Image and ImageJ software have been pioneers as open tools for the analysis of scientific images. We discuss the origins, challenges and solutions of these two programs, and how their history can serve to advise and inform other software projects.

Conflict of interest statement

Competing financial interests

The authors declare no competing financial interests.


Figure 1
Figure 1
Appearance of NIH Image and ImageJ. Screenshots of NIH Image in 199X (a) and ImageJ 1.45s in 2012 (b). Note that while the look and feel is slightly different, the overall feature layout and menu structure is basically the same.
Figure 2
Figure 2
ImageJ plugins bundled with each ImageJ release over time.

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