[Effect of hydrogen inhalation on p38 MAPK activation in rats with lipopolysaccharide- induced acute lung injury]

Nan Fang Yi Ke Da Xue Xue Bao. 2012 Aug;32(8):1211-3.
[Article in Chinese]


Objective: To investigate the effect of hydrogen inhalation on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and the underlying molecular mechanisms.

Methods: Thirty-two male SD rats were randomly divided into 4 groups (n=8), namely the normal saline group (SA), saline with 2% hydrogen gas inhalation group (SH group), ALI group, and ALI with hydrogen inhalation group (LH group). In the two ALI groups, ALI was induced by intraperitoneal injection of 15 mg/kg LPS. Treatments with inhalation of 2% hydrogen gas for 6 h was administered after the injection of LPS or saline. The concentrations of tumor necrosis factor-α (TNF-α) in the lung tissue and serum were examined with ELISA. The expression of p38 MAPK in the lung tissue was detected by Western blotting..

Results: Hydrogen inhalation decreased the expression of p-p38 MAPK in the lung tissue, and significantly reduced TNF-α content in the lung tissue and serum of rats with ALI.

Conclusion: Hydrogen inhalation can decrease the expression of TNF-α in the lung tissue and serum, and this effect may be related with reduced p38 MAPK expression and inhibition of p38 MAPK activation.

Publication types

  • English Abstract

MeSH terms

  • Acute Lung Injury / blood
  • Acute Lung Injury / chemically induced
  • Acute Lung Injury / metabolism*
  • Administration, Inhalation
  • Animals
  • Hydrogen / administration & dosage*
  • Lipopolysaccharides
  • Lung / metabolism*
  • Male
  • Rats
  • Rats, Sprague-Dawley
  • Tumor Necrosis Factor-alpha / blood
  • Tumor Necrosis Factor-alpha / metabolism*
  • p38 Mitogen-Activated Protein Kinases / blood
  • p38 Mitogen-Activated Protein Kinases / metabolism*


  • Lipopolysaccharides
  • Tumor Necrosis Factor-alpha
  • Hydrogen
  • p38 Mitogen-Activated Protein Kinases