Rapid determination of lymphogranuloma venereum serovars of Chlamydia trachomatis by quantitative high-resolution melt analysis (HRMA)

Jimmy Twin; Matthew P Stevens; Suzanne M Garland; Angelo M Zaia; Sepehr N Tabrizi

doi:10.1128/JCM.01670-12

Rapid determination of lymphogranuloma venereum serovars of Chlamydia trachomatis by quantitative high-resolution melt analysis (HRMA)

J Clin Microbiol. 2012 Nov;50(11):3751-3. doi: 10.1128/JCM.01670-12. Epub 2012 Aug 29.

Authors

Jimmy Twin¹, Matthew P Stevens, Suzanne M Garland, Angelo M Zaia, Sepehr N Tabrizi

Affiliation

¹ Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Melbourne, Australia. jimmy.twin@mcri.edu.au

Abstract

A quantitative high-resolution melt analysis assay was developed to differentiate lymphogranuloma venereum-causing serovars of Chlamydia trachomatis (L1 to L3) from other C. trachomatis serovars (D to K). The detection limit of this assay is approximately 10 copies per reaction, comparable to the limits of other quantitative-PCR-based methods.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Bacteriological Techniques / methods*
Chlamydia trachomatis / classification*
Chlamydia trachomatis / genetics
Chlamydia trachomatis / isolation & purification*
Humans
Lymphogranuloma Venereum / microbiology*
Male
Molecular Diagnostic Techniques / methods*
Sensitivity and Specificity
Transition Temperature