Destabilized eYFP variants for dynamic gene expression studies in Corynebacterium glutamicum

Microb Biotechnol. 2013 Mar;6(2):196-201. doi: 10.1111/j.1751-7915.2012.00360.x. Epub 2012 Sep 3.

Abstract

Fluorescent reporter proteins are widely used for the non-invasive monitoring of gene expression patterns, but dynamic measurements are hampered by the extremely high stability of GFP and homologue proteins. In this study, we used SsrA-mediated peptide tagging for the construction of unstable variants of the GFP derivative eYFP (enhanced yellow fluorescent protein) and applied those for transient gene expression analysis in the industrial platform organism Corynebacterium glutamicum.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Biotechnology / methods*
  • Corynebacterium glutamicum / genetics
  • Corynebacterium glutamicum / metabolism*
  • Gene Expression Regulation, Bacterial*
  • Genes, Reporter
  • Genetic Variation*
  • Genetic Vectors
  • Luminescent Proteins / chemistry
  • Luminescent Proteins / genetics*
  • Luminescent Proteins / metabolism
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • Protein Stability*
  • RNA-Binding Proteins / chemistry
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism

Substances

  • Bacterial Proteins
  • Luminescent Proteins
  • RNA-Binding Proteins
  • small protein B
  • yellow fluorescent protein, Bacteria