In vitro antiviral activity of sulfated Auricularia auricula polysaccharides

Carbohydr Polym. 2012 Oct 15;90(3):1254-8. doi: 10.1016/j.carbpol.2012.06.060. Epub 2012 Jun 29.


Total Auricularia auricula polysaccharide (AAP(t)) was prepared by extracting and removing the proteins. Column chromatography was used to further graded it into AAP(1) and AAP(2). Three AAPs were modified by chlorosulfonic acid-pyridine method to obtain three sulfated AAPs (sAAPs), sAAP(t), sAAP(1) and sAAP(2), respectively. Three sAAPs and Newcastle disease virus (NDV) were added into cultivation system of chicken embryo fibroblast (CEF) in three manners, pre-, post- and simultaneous-adding polysaccharide with NDV respectively, taking three non-modified AAPs as control. Their anti-viral activities were compared by MTT method. The results showed that sAAPs and AAPs at a certain concentration could significantly inhibit the cellular infectivity of NDV in three manners. The effects of sAAPs were better than that of AAPs. It indicated that sulfated modification could enhance the antiviral activity of AAP. sAAP(1) and sAAP(t) possessed stronger activity and would be as the component of a new-type antiviral drug.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agaricales / chemistry*
  • Animals
  • Antiviral Agents / chemistry
  • Antiviral Agents / pharmacology*
  • Cells, Cultured
  • Chick Embryo
  • Dose-Response Relationship, Drug
  • Fibroblasts / pathology
  • Fibroblasts / virology*
  • Fungal Polysaccharides / chemistry
  • Fungal Polysaccharides / pharmacology*
  • Newcastle Disease / drug therapy*
  • Newcastle Disease / pathology
  • Newcastle Disease / virology
  • Newcastle disease virus*


  • Antiviral Agents
  • Fungal Polysaccharides