Reduced exposure evaluation of an Electrically Heated Cigarette Smoking System. Part 1: Non-clinical and clinical insights

Regul Toxicol Pharmacol. 2012 Nov 1;64(2 Suppl):S1-10. doi: 10.1016/j.yrtph.2012.08.008. Epub 2012 Aug 23.


The following series of papers presents an extensive assessment of the Electrically Heated Cigarette Smoking System EHCSS series-K cigarette vs. conventional lit-end cigarettes (CC) as an example for an extended testing strategy for evaluation of reduced exposure. The EHCSS produces smoke through electrical heating of tobacco. The EHCSS series-K heater was designed for exclusive use with EHCSS cigarettes, and cannot be used to smoke (CC). Compared to the University of Kentucky Reference Research cigarette 2R4F and a series of commercial CC, mainstream cigarette smoke of both the non-menthol and menthol-flavored EHCSS cigarettes showed a reduced delivery of a series of selected harmful and potentially harmful constituents (HPHC), mutagenic activity determined using the Salmonella typhimurium Reverse Mutation (Ames) assay, and cytotoxicity in the Neutral Red Uptake Assay. Clinical evaluations confirmed reduced exposure to HPHC and excretion of mutagenic material under controlled clinical conditions. Reductions in HPHC exposure were confirmed in a real-world ambulatory clinical study. Potential biomarkers of cardiovascular risk were also reduced under real-world ambulatory conditions. A modeling approach, 'nicotine bridging', was developed based on the determination of nicotine exposure in clinical evaluations which indicated that exposure to HPHC for which biomarkers of exposure do not exist would also be reduced.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers / analysis
  • Biomarkers / urine
  • Carbon Monoxide / chemistry
  • Carbon Monoxide / toxicity
  • Clinical Trials as Topic
  • Electricity
  • Humans
  • Inhalation Exposure / adverse effects
  • Inhalation Exposure / analysis
  • Inhalation Exposure / prevention & control*
  • Mutagenicity Tests
  • Mutagens / chemistry
  • Mutagens / toxicity*
  • Nicotine / chemistry
  • Nicotine / toxicity
  • Salmonella typhimurium / drug effects
  • Salmonella typhimurium / genetics
  • Tars / chemistry
  • Tars / toxicity
  • Tobacco / chemistry
  • Tobacco / toxicity*
  • Tobacco Products / analysis*
  • Tobacco Products / toxicity
  • Tobacco Smoke Pollution / adverse effects
  • Tobacco Smoke Pollution / analysis
  • Tobacco Smoke Pollution / prevention & control*


  • Biomarkers
  • Mutagens
  • Tars
  • Tobacco Smoke Pollution
  • tobacco tar
  • Nicotine
  • Carbon Monoxide